Ppt micropropagation powerpoint presentation, free. Explant cell, tissue or organ of a plant that is used to start in vitro cultures. Agara polysaccharide powder derived from algae used to gel a medium. Micropropagation procedure is divided in stages for the sake of understanding. Within a production period less than 4 months, a commercial laboratory can obtain. In protocols for micropropagation of selected economicallyimportant horticultural plants, well recognised researchers in the field compile stepwise protocols for rapid plant multiplication of economicallyimportant horticultural species.
Ppt micropropagation powerpoint presentation free to. Tissue culture allows the production and propagation of genetically homogeneous, diseasefree plant material 37. Cell and tissue in vitro culture is a useful tool for the induction of somaclonal variation 38. Micropropagation enables growers to increase the production of plants that normally propagate very slowly such as narcissus and other bulbous crops. Definition of micropropagation clonal propagation in vitro micro propagation is the production of whole plant from small section of plant such as a stem tip, node, meristem, embryo, or even a seed. Heritage plant conservation through micropropagation is the property of its rightful owner. This is particularly valuable in the case of difficultto.
Micropropagation definition of micropropagation by the. The history of micropropagation advantages of micropropagation five stages of shoot culture video video dr. Pdf studies on micropropagation of pineapple ananas. Plant tissue culture, cell culture or micropropagation is the technique of producing selected plants of known desirable agriculture qualities, in large numbers of plants from small pieces of plant in relatively short period times. Micropropagation is the production of new plants under the controlled environment within. Plant tissue culture micropropagation ppt by deepak chaudhari authorstream presentation. Meristem micropropagation of cassava manihot esculenta. Clone genetically identical assemblage of individuals propagated entirely by vegetative means from a single plant. In vitro micropropagated samples were obtained from the tissue culture facility of the mikocheni agriculture research institute mari dar es salaam, tanzania and were propagated as described by konan et al. Micropropagation is a highly accepted technology for commercial exploitation.
If so, share your ppt presentation slides online with. With orchids it is the only way to reproduce the plants as. Many ornamental plants and woody species are propagated commercially by shoot tip and axillary bud proliferation. Tissue culture involves the use of small pieces of plant tissue explants which are cultured in a. Micropropagation is the truetotype propagation of a selected genotype using in vitro culture techniques. Acclimatization of plants transferred to soil micropropagation successful plant transfer from culture to soil show high survival rate and cost involved in the process is low. The potato micropropagation armstatehydromet and ministry of agriculture yerevan, march 2012 rolot jeanlouis. Apr 16, 20 for more information, log on to download the study materials here moder. It is also used to provide a sufficient number of plantlets for planting from a stock plant. Micropropagation is the practice of rapidly multiplying stock plant material to produce many progeny plants, using modern plant tissue culture methods micropropagation is used to multiply plants such as those that have been genetically modified or bred through conventional plant breeding methods. Murashige proposed three i to iii stages, debergh and maene added stage 0. A revised medium for rapid growth and bioassays with tobacco tissue. Biotechnology has been introduced into agricultural practice at a rate without precedent. Virus free plant sub millimeter portion of shoottip stock plant virus free nodal cutting disadvantage of using small sized explant for micropropagation shoot tip explant low survival rate and slow initial growth.
Find powerpoint presentations and slides using the power of, find free presentations research about micropropagation ppt. Theyll give your presentations a professional, memorable appearance the kind of sophisticated look that todays audiences expect. Shoot culture 9most widely used method for commercial micropropagation 9relatively high genetic stability in the plants produced shoot culture advantages 9reliable rates and consistency of shoot multiplication 93 8 fold multiplication rate per month 9preexisting meristems are least susceptible to genetic changes micropropagation stages stage 0. Agar is generally used at a concentration of 612 gliter. Micropropagation definition of micropropagation by the free.
Protocols for micropropagation of selected economically. Worlds best powerpoint templates crystalgraphics offers more powerpoint templates than anyone else in the world, with over 4 million to choose from. Unit ii biotechnology and plants lesson 7 plant tissue culture 169 cells and tissue the starting point for micropropagation can be individual cells or small amounts of plant tissue. Micropropagation plants can be propagated by sexual through generation of seeds or asexual through multiplication of vegetative parts means. Transplanted plantlets shoot immediately irrigated with inorganic nutrient solution and maintained under high humidity for initial 1015 days. There is great interest in the phenotypic, genetic and epigenetic changes associated with plant in vitro culture known as somaclonal variation. Mike demonstration of sterile technique to divide micropropagules agristarts, commercial micropropagation kane web lecture d w vd objectives are to understand r. A free powerpoint ppt presentation displayed as a flash slide show on id. In this chapter various aspects of micropropagation have been discussed. Recent studies2 4 revealed that most chlorophyllous plants in vitro have the ability to grow photoautotrophically, and that the low co 2 concentration in the airtight. Most often micropropagation is also associated with mass production at a competitive price. A read is counted each time someone views a publication summary such as the title, abstract, and list of authors, clicks on a figure, or views or downloads the fulltext. Now a days there are many laboratories which produce and export all types of desired plants through this technique. Micropropagation techniques such as clonal propagation of nodal segments and microtuberization yields high quality and pathogenfree potato plants and miniaturized tuber seeds, also known as microtubers.
Aug 03, 2017 micropropagation plants can be propagated by sexual through generation of seeds or asexual through multiplication of vegetative parts means. Micropropagation differs from all other conventional propagation methods in that aseptic conditions are essential to achieve success. Plant biotech page 2 plant biotechnology field of dreams the field of plant biotechnology is concerned with developing ways to improve the production of plants in. Clonal propagation refers to the process of asexual reproduction by multiplication of genetically identical copies of individual plants, where the term clone is used to represent a plant population. Currently we have accepted five stages procedure 0 to iv. View and download powerpoint presentations on micropropagation ppt. Tissue culture involves the use of small pieces of plant tissue explants which are cultured in a nutrient medium under sterile conditions. Plant tissue culture micropropagation ppt by deepak. Vegetative propagation of sterile hybrids can be used as parent plants for seed production.
For more information, log on to download the study materials here moder. Fileplanet microsoft powerpoint 2016 free download. Jan 28, 2017 plant tissue culture sample in a test tube. Winner of the standing ovation award for best powerpoint templates from presentations magazine. Micropropagation is the practice of rapidly multiplying stock plant material to produce many progeny plants, using modern plant tissue culture methods.
Plant tissue culture micropropagation ppt by deepak chaudhari. Axillary bud proliferation typically results in an average tenfold increase in shoot number per monthly culture passage. In plants, the dna of interest is transferred into the new plant by using agrobacterium tumefaciens, a bacterium that can infect plant tissues and incorporate part of. The present study was carried out to establish the micropropagation protocols for use in large scale propagation and the development of an in vitro regeneration system for the use in genetic. Us20020086425a1 micropropagation, synthetic seeds and. Page advantages of micropropagation includes merits, demerits and problems interfering during micropropagation. Studies on micropropagation of pineapple ananas comosus l. A tissue culture technique for plant propagation in which tissue is taken from a plant and grown in a laboratory to produce plantlets that are.
It is done under sterile conditions in a laboratory using plant seed or tissue. When comparing micropropagation to traditional cloning or seed propagation, the cost benefits of. Protocol for micropropagation of wood y trees and fruits. Introduction of disease free new cultivars is possible through micropropagation 6. Micropropagation is an advanced vegetative propagation technology for producing a large number of genetically superior and pathogenfree transplants in a limited time and space. A variety of conditions including type of medium, concentrations of auxin and cytokinins, temperature, lighting, humidity and sterilization are disclosed, the use of which results in a high percentage success rate of micropropagation thereby allowing economically viable production of bamboo on a large or mass scale. Plant cells for micropropagation may be newly transformed as a result of genetic engineering, or they might be cells taken from plant tissue. Project on micropropagation free download as powerpoint presentation.
Create or view powerpoint presentations with this uptodate version of the popular software package. In the first step cultures were initiated on msi media fortified with 0. In vitro propagation systems that are based on the use of microcuttings or meristem cultures are considered analogous to clonal cuttings and so widely viewed to be largely free from such somaclonal effects. Tissue culture serves as an alternative to vegetative propagation by conventional methods, such as by rooting of cuttings. In nature, clonal propagation occurs by apomixis seed development without meiosis and fertilization andor vegetative propagation regeneration of new plants from vegetative parts. Read this article to learn about the techniques, factors, applications and disadvantages of micro propagation process and also about production of diseasefree plants and its merits and demerits. Unit ii biotechnology and plants plant tissue culture 7. When comparing micropropagation to traditional cloning or seed propagation, the cost benefits of transitioning to tissue culture become evident. Micropropagation micropropagation is the production of whole plants from small sections of plant such as a shoot tip, node, meristem, embryo, or even a seed plant tissue culture is basically the same thing, except that it implies the use of callus tissue generated from plant cells cultured invitro. A piece of plant tissue called an explant is a cut from the plant, b disinfested removal of surface contaminants, and c.
Plants can be propagated by sexual through generation of seeds or asexual through multiplication of vegetative parts means. Micropropagation of orchids micropropagation is the process used to replicate plants such as orchids, the word micropropagation means simply propagation in miniature. Explants carrying performed vegetative bud suitable for enhanced axillary branching. The invention consists of these novel solutions both individually and in combination. Danguole kamstaityte, vidmantas stanys lithuanian institute of horticulture, babtai lt54333, kaunas distr. Plant tissue culture terminology adventitiousdeveloping from unusual points of origin, such as shoot or root tissues, from callus or embryos, from sources other than zygotes. Threestage protocol has been developed for rapid mass propagation of pineapple ananas comosus cv. Ppt micropropagation powerpoint presentation, free download. The problem areas addressed by the various aspects of the invention include the following. The process of micropropagation can be divided into four stages.167 1005 727 851 622 497 613 221 1371 858 1288 1566 1515 246 1166 475 224 260 538 853 1399 457 1414 240 144 473 1252 436 1385 931 519 679 540 1124 1360 1321 1194 776 1449 72 874